aav8 esyn dio enphr3 0 egfp Search Results


aav8 esyn dio enphr3 0 egfp  (Vector Biolabs)
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Vector Biolabs aav8 esyn dio enphr3 0 egfp
a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received <t>AAV8-Syn-DIO-eNpHR3.0-EGFP</t> virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.
Aav8 Esyn Dio Enphr3 0 Egfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.

Journal: bioRxiv

Article Title: Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence

doi: 10.1101/2020.07.02.183780

Figure Lengend Snippet: a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.

Article Snippet: Mice were injected in each side or one side of the DMH with ∼0.5 µL AAV2-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, Cambridge, USA), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (Addgene), AAV8-eSyn-DIO-EGFP (Addgene) or AAV8-eSyn-DIO-eNpHR3.0-EGFP (Vector Biolabs, Malvern, PA, USA) using the following coordinates: AP: −1.85 mm, L: ± 0.3 mm, DV: −5.5 mm.

Techniques: Plasmid Preparation, Construct, Injection, Expressing